产品分类
联系方式
- 联系人:吴海燕
- 电 话:13651665725
- 传 真:3001028570@qq.com
- 邮 箱:info@zzsrm.com
- 手 机:13651665725
- 地 址:上海市闵行区浦江镇联航路1588号产业大楼701室（手机 19921389057）
|
 |
- 品牌：Megazyme
- 产地：爱尔兰
- 型号：1kit
- 纯度：99%
- 发布日期： 2013-12-24
- 更新日期： 2024-04-18
品牌：
Megazyme
产品货号： K-ACETAK
品牌： Megazyme
产品品名：乙酸【 AK 法】检测试剂盒
英文品名： ACETIC ACID(ACETATE KINASE FORMAT) assay Kit
规格型号： 550 次（ 170.5 ml ）
Analyser format for the specific assay of acetic acid (acetate) inbeverages and food products. On calibration, the prepared reagent islinear to > 28 micrograms of acetic acid per mL of assay solution.
INTRODUCTION:
The most widely used method for enzymatic quantification of acetic
acid is that employing acetyl-coenzyme A synthetase (ACS). However,
this method is based on the use of an indicator reaction catalysed by
L-malate dehydrogenase that is in permanent equilibrium, and therefore
a non-stoichiometric increase in absorbance is observed from the
acetate present in the sample. Thus, a slightly non-linear response
to increasing acetate concentration is observed upon calibration,
resulting in poor R2 values. In addition (depending on the supplier)
reagents prepared for auto-analyser applications can have very limited
on-machine stability, due to rapidly increasing blank absorbance values.
To overcome these issues, Megazyme developed this alternative acetic
acid kit, based on the enzyme acetate kinase (AK; see equations 1-3
below), especially for the auto-analyser user. This reagent has improved
on-machine stability, gives excellent linear calibration curves, and results
in a stoichiometric change in absorbance due to the acetic acid present
in the sample.
KITS:
Kits suitable for performing a minimum of 550 assays in auto-analyser
format are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (11 mL, pH 7.4) plus sodium azide (0.02% w/v ) as a
preservative. Stable for > 2 years at 4 ° C.
Bottle 2: NADH plus ATP, PEP and PVP.
Stable for > 2 years at either 4 ° C or -20 ° C.
Bottle 3: Acetate kinase plus pyruvate kinase and D-lactate
dehydrogenase suspension, 4.1 mL.
Stable for > 2 years at 4 ° C.
温馨提示：需要详细的产品使用说明书，请联系甄准生物：
联系人：杨树彦
电话： 021-68404353
传真： 021-58979353
手机 /QQ ： 13795317828
