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- 品牌:Megazyme
- 产地:爱尔兰
- 型号:1kit
- 纯度:99%
- 发布日期: 2013-12-24
- 更新日期: 2024-05-11
品牌:
Megazyme
产品货号: K-GLUT
品牌: Megazyme
产品品名: L- 谷氨酸检测试剂盒
英文品名: L-Glutamic Acid Assay Kit
规格型号: 60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)
A simple, reliable, rapid and accurate method for the determination ofL-glutamate (MSG) in foodstuffs.
INTRODUCTION:
L-Glutamic acid (L-glutamate), one of the 20 common amino acids,
occurs naturally in foods such as cheese, milk, meat, fish, corn,
tomatoes, mushrooms, soybean and sugar beet. In its free form,
L-glutamic acid is the major flavour enhancing component of foods
with a meaty/savoury taste. The role played by L-glutamic acid in
the palatability of foods is exploited by either traditional cooking
methods that release free L-glutamic acid, or by supplementation
with monosodium glutamate (MSG). Excessive use of MSG as a
food additive (E621) in processed foods results in the symptoms of
“ Chinese Restaurant Syndrome ” (CRS), where palpitations, severe
headaches, nausea, wheezing and increased body temperature are
thought to result from the neuro-excitory properties of free
L-glutamic acid. Onset of CRS is fast, only 30-60 min after ingestion,
and symptoms generally subside after approximately 2-3 hours.
Around 15% of Americans are sensitive to MSG.
KITS:
Kits suitable for performing 60 assays in manual format (or 700 assays
in auto-analyser format or 600 assays in microplate format) are available
from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (35 mL, pH 8.6) plus sodium azide (0.02% w/v)
as a preservative.
Stable for > 2 years at 4 ° C.
Bottle 2: (x2) NAD+ plus INT.
Stable for > 2 years at 4 ° C.
Bottle 3: Diaphorase suspension (3.1 mL).
Stable for > 2 years at 4 ° C.
Bottle 4: Glutamate dehydrogenase solution (3.1 mL).
Stable for > 2 years at -20 ° C.
Bottle 5: L-Glutamic acid standard solution
(5 mL, 0.10 mg/mL) in 0.02% (w/v) sodium azide.
Stable for > 2 years at 4 ° C.
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