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- 品牌：Megazyme
- 产地：爱尔兰
- 型号：1kit
- 纯度：99%
- 发布日期： 2013-12-24
- 更新日期： 2024-05-11
品牌：
Megazyme
产品货号： K-FRGLMQ
品牌： Megazyme
产品品名： D- 果糖 D- 葡萄糖检测试剂盒
英文品名： D-Fructose and D-Glucose MegaQuant? Format Assay Kit
规格型号： 60 assays per kit
For the measurement of D-fructose and D-glucose in grapes, grape juiceand wine using the MegaQuant? colorimeter (measurement at 505 nm).
INTRODUCTION:
D-Fructose and D-glucose are found in most plant products. In foods,they are present in significant quantities in honey, wine and beer, anda range of solid foodstuffs such as bread and pastries, chocolate andcandies. In the wine industry, the D-fructose and D-glucose content isone of the most important parameters and is monitored at each stage ofthe wine making process. The specific aim of the current assay format isto provide a simple, rugged, reliable and accurate method for themeasurement of D-fructose plus D-glucose employing an inexpensivecolorimeter (the MegaQuantTM Meter).
KITS:
Kits suitable for performing 60 determinations are available fromMegazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (100 mL, pH 10.0) plus sodium azide (0.02% w/v) as a preservative.
NOTE: This buffer contains sodium azide (0.02% w/v) as a
preservative, and thus should not be dispensed using a mouth pipette.
Stable for > 2 years at 4 ° C.
Bottle 2: Test tablets (60) containing NADP+, ATP, INT and FAD. Theseare supplied in a special plastic vial containing impregnated desiccant.Allow this container to warm to room temperature (preferably in thepresence of a desiccant) before opening to remove tablets. This willensure that the remaining tablets will not absorb moisture and thusguarantee maximum stability. Stable for > 2 years when stored at-20 ° C in a domestic freezer (preferably in a sealed container with adrying agent such as silica gel).
Bottle 3: Hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoseisomerase and diaphorase suspension, 1.3 mL.
Stable for
> 2 years at 4 ° C.
Bottle 4: D-Fructose standard solution (5 mL, 0.50 mg/mL) in 0.02% w/vsodium azide.
Stable for
> 2 years at 4 ° C.
Bottle 5: Tablets (65) containing PVPP.
Stable for
> 5 years at room temperature.
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