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品牌：
Megazyme
产品货号：K-BGLU
品牌：Megazyme
产品品名：β-葡聚糖[混联]检测试剂盒
英文品名：β-Glucan (Mixed Linkage) Assay Kit
规格型号：100 assays per kit
AACC Method 32-23.01, AOAC Method 995.16, EBC Methods 3.11.1, 4.16.1and 8.11.1, ICC Standard No. 166 and RACI standard method for themeasurement of 1,3:1,4-?-D-glucan in cereal grains, milling fractions,wort, beer and other food products. Recommended/Standard procedure ofthe Association of Official Analytical Chemists (AOAC), AmericanAssociation of Cereal Chemists (AACC), Royal Australian ChemicalInstitute (RACI) and International Association for Cereal Science andTechnology (ICC).
INTRODUCTION:
For some time the brewing, food and ingredient industries have
identified the need to develop an accurate, convenient and reliable
method for assaying mixed-linkage β-glucan in barley, malt, wort
and beer. The Megazyme method meets all of these requirements.
It is simple to use and fifty to one hundred (50-100) samples can
be assayed in a day. The method has now been adapted for the
measurement of β-glucan in oats and oat fibre products (see page 10).
KITS:
Kits suitable for performing 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Lichenase [specific, endo-(1-3)(1-4)-β-D-glucan
4-glucanohydrolase] suspension (1 mL).
Stable for > 3 years at 4°C.
Bottle 2: β-Glucosidase (1 mL) suspension.
Stable for > 3 years at 4°C.
Bottle 3: GOPOD Reagent Buffer. Buffer (48 mL,
pH 7.4), p-hydroxybenzoic acid and sodium azide
(0.4 % w/v).
Stable for > 4 years at 4°C.
1
Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase
plus peroxidase and 4-aminoantipyrine. Freeze-dried
powder.
Stable for > 5 years at -20°C.
Bottle 5: D-Glucose standard solution (5 mL, 1.0 mg/mL) in
0.2 % (w/v) benzoic acid.
Stable for > 5 years at room temperature.
Bottle 6: Standardised barley flour control. β-Glucan content
shown on vial label.
Stable for > 5 years at room temperature.
Bottle 7: Standardised oat flour control. β-Glucan content
shown on vial label.
Stable for > 5 years at room temperature.
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