产品货号：MGZ-K-AMIAR

产品品名：氨（快速）检测试剂盒

英文品名：Ammonia (Rapid) assay Kit

规格型号：96 assays (manual) /960 assays (microplate) / 960 assays (auto-analyser) 

For the rapid assay of ammonia in all samples, including grape juice and wine (and other foods/beverages).Suitable for manual, auto-analyser and microplate formats.

INTRODUCTION:

Ammonia is a widely occurring natural compound, often produced as

a consequence of microbial protein catabolism, and thus serves as a

quality indicator of fruit juice, milk, cheese, meat, seafood and bakery

products. Unlike some other kits, this kit benefits from the use of a

gluta mate dehydrogenase that is not inhibited by tannins found in, for

example, grape juice and wine. K-AMIAR can be used to determine

ammonia manually (see page 4, “A”) or in auto-analyser format

(see page 6, “B”). In the wine industry, ammonia determination is

important in the calculation of yeast available nitrogen (YAN). YAN

comprises three highly variable components, free ammonium ions,

primary amino nitrogen (from free amino acids), and the contribution

from the side chain of L-ar ginine1. Thus, for the most accurate

determination of YAN, all three components should be quantified,

and this is possible using Megazyme’s L-Arg inine/Urea/Ammonia kit

(K-LARGE) and NOPA kit (K-PANOPA).

KITS:

Kits suitable for performing 96 assays in manual format (or 960 assays

in auto-analyser format or 960 assays in microplate format) are

available from Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (36 mL, pH 8.0) plus 2-oxoglutarate and

sodium azide (0.02% w/v) as a preservative.

Stable for 大于 2 years at 4°C.

Bottle 2: (x 2) NADPH.

Stable for 大于 5 years at -20°C.

Bottle 3: Gluta mate dehydrogenase suspension (2.2 mL).

Stable for 大于 2 years at 4°C.

3

Bottle 4: Ammonia standard solution (5 mL, 0.04 mg/mL) in

0.02% (w/v) sodium azide.

Stable for 大于 2 years at 4°C.

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