和光WAKO基因重组蛋白精制用载体--标签抗体及相关试剂

   重组蛋白标签（Recombinant Protein Tags）是指利用DNA体外重组技术，与目的蛋白一起融合表达的一种多肽或者蛋白，以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展，研究人员相继开发出了具有各种不同功能的蛋白标签。目前，根据上海甄准生物研究统计，这些蛋白标签已在基础研究和商业化产品生产等方面得到了广泛的应用。

      Flag标签蛋白为编码8个氨基酸的亲水性多肽（DYKDDDDK），同时载体中构建的Kozak序列使得带有FLAG的融合蛋白在真核表达系统中表达效率更高。 FLAG作为标签蛋白，其融合表达目的蛋白后具有以下优点：

FLAG作为融合表达标签，其通常不会与目的蛋白相互作用并且通常不会影响目的蛋白的功能、性质，这样就有利用研究人员对融合蛋白进行下游研究。

融合FLAG的目的蛋白，可以直接通过FLAG进行亲和层析，此层析为非变性纯化，可以纯化有活性的融合蛋白，并且纯化效率高。

FLAG作为标签蛋白，其可以被抗FLAG的抗体识别，这样就方便通过Western Blot、ELISA等方法对含有FLAG的融合蛋白进行检测、鉴定。

融合在N端的FLAG，其可以被肠激酶切除（DDDK），从而得到特异的目的蛋白。因此现FLAG标签已广泛的应用于蛋白表达、纯化、鉴定、功能研究及其蛋白相互作用等相关领域。

    标签蛋白抗体(Tag-antibody)可用于检测各种商品化表达载体上的标签序列（如：DYKDDDDK, 6xHis, GFP, Myc，GST、HA等），籍以分析鉴定目的蛋白的表达含量及其功能。其原理是抗原—抗体反应，更多技术相关信息可以咨询上海甄准生物科技有限公司，这些标签抗体可以高度特异地结合对应的标签融合蛋白，从而达到对相应的标签融合表达对蛋白的表达、细胞内定位，以及纯化、定性或定量的研究。

The DYKDDDDK was consisted of 8 amino acids and has been used as the tag for detection and/or purification of recombinant protein.

Monoclonal anti DYKDDDDK tag antibody（018-22381,014-22383,012-22384）was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic DYKDDDDK peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites.（This product is for research use only. Do not administer it to human.）

Anti DYKDDDDK tag Beads（012-22781,018-22783,016-22784） is the slurry of anti DYKDDDDK monoclonal antibody immobilization beads. This product is used for the purification of DYKDDDDK tagged recombinant proteins by the competitive elution of DYKDDDDK peptide.[Setting Volume] 1.8-2.1ml slurry/ml Beads.

This product is used for detection of GST fusion recombinant protein by using immunoprecipitation and western blot.

Anti Glutathione S-transferase, Monoclonal Antibody（013-21851,013-21856,017-21854） is for research use only. Do not administer it to human.

Glutathione S-transferase (GST) has been used as the tag for detection and/or purification of recombinant protein.

Monoclonal Anti GST antibody was produced by hybridoma clone 5A7. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with GST protein. This antibody was purified from mouse ascites.

Hemagglutinin (HA) is an antigenic glycoprotein found on the surface of influenza viruses and many other bacteria and viruses.

The partial peptide of HA protein which consists of 9 amino acids, YPYDVPDYA, has been used as the tag for detection and/or purification of recombinant protein.

Monoclonal Anti HA antibody was produced by hybridoma clone 4B2. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic YPYDVPDYA peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites.