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K-BGLU,β-葡聚糖[混联]检测试剂盒,β-Glucan (Mixed Linkage) Assay Kit

发表时间:2014-06-13

  K-BGLU,β-葡聚糖[混联]检测试剂盒,β-Glucan (Mixed Linkage) Assay Kit

产品识别号:K-BGLU

产品品名:β-葡聚糖[混联]检测试剂盒

英文品名:β-Glucan (Mixed Linkage) Assay Kit

规格型号:100 assays per kit

AACC Method 32-23.01, AOAC Method 995.16, EBC Methods 3.11.1, 4.16.1and 8.11.1, ICC Standard No. 166 and RACI standard method for themeasurement of 1,3:1,4-?-D-glucan in cereal grains, milling fractions,wort, beer and other food products. Recommended/Standard procedure ofthe Association of Official Analytical Chemists (AOAC), AmericanAssociation of Cereal Chemists (AACC), Royal Australian ChemicalInstitute (RACI) and International Association for Cereal Science andTechnology (ICC).

INTRODUCTION:

For some time the brewing, food and ingredient industries have

identified the need to develop an accurate, convenient and reliable

method for assaying mixed-linkage β-glucan in barley, malt, wort

and beer. The Megazyme method meets all of these requirements.

It is simple to use and fifty to one hundred (50-100) samples can

be assayed in a day. The method has now been adapted for the

measurement of β-glucan in oats and oat fibre products (see page 10).

KITS:

Kits suitable for performing 100 assays are available from Megazyme.

The kits contain the full assay method plus:

Bottle 1: Lichenase [specific, endo-(1-3)(1-4)-β-D-glucan

4-glucanohydrolase] suspension (1 mL).

Stable for > 3 years at 4°C.

Bottle 2: β-Glucosidase (1 mL) suspension.

Stable for > 3 years at 4°C.

Bottle 3: GOPOD Reagent Buffer. Buffer (48 mL,

pH 7.4), p-hydroxybenzoic acid and sodium azide

(0.4 % w/v).

Stable for > 4 years at 4°C.

1

Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase

plus peroxidase and 4-aminoantipyrine. Freeze-dried

powder.

Stable for > 5 years at -20°C.

Bottle 5: D-Glucose standard solution (5 mL, 1.0 mg/mL) in

0.2 % (w/v) benzoic acid.

Stable for > 5 years at room temperature.

Bottle 6: Standardised barley flour control. β-Glucan content

shown on vial label.

Stable for > 5 years at room temperature.

Bottle 7: Standardised oat flour control. β-Glucan content

shown on vial label.

Stable for > 5 years at room temperature.

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