K-DMAL，D-苹果酸检测试剂盒，D-Malic Acid Assay Kit
产品识别号：K-DMAL
产品品名：D-苹果酸检测试剂盒
英文品名：D-Malic Acid Assay Kit
规格型号：100 assays (manual) / 1000 assays (microplate) / 1100 assays(auto-analyser) per kit
For the specific assay of D-malic acid (D-malate) in beverages and food products.
INTRODUCTION:
In Nature, essentially all malic acid is produced in the L-racemic
form (the exception being a few micro-organisms which produce
D-malic acid). Freshly pressed fruit juices contain D-malic acid at
the detection limit (~ 10 mg/L). Malic acid is produced commercially
in the D-/L-racemic mixture by the catalytic hydration of maleic
anhydride and can replace citric acid in food products as an acidulant
and flavour enhancer. It finds application in flavoured fruit drinks,
juices and wines. The detection of D-malic acid in juices or wine
indicates that it has been added. The legal situation concerning the
addition of D-/L-malic acid to juice or wine varies between countries.
KITS:
Kits suitable for performing 100 assays in manual format (or 1100 assays
in auto-analyser format or 1000 assays in microplate format) are available
from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 8.0) plus sodium azide (0.02% w/v)
as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+.
Stable for > 5 years at -20°C.
Bottle 3: D-Malate dehydrogenase suspension (2.2 mL).
Stable for > 2 years at 4°C.
Bottle 4: D-Malic acid standard solution (5 mL, 0.20 mg/mL).
Stable for > 2 years at room temperature.
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